Haematological Research Centre of the Russian Academy of Medical Sciences
In order to study the influence of water exposed in a pyramid on the coagulatory system of the blood we carried out a study of the influence of this substance on certain coagulatory parameters in vitro using donor blood as well as standard normal and pathological (reduced level of coagulatory factors) lyophilised human plasma and lyophilised pathological human plasma deficient in factor VIII. Besides this we made a study of pyramid water in vivo, in an experiment with rabbits.
The donor blood was obtained by puncturing the cubital vein and stabilised with a 3.8% solution of sodium citrate (Merck, Germany) in a ratio of 9:1. Blood samples for the evaluation of coagulatory parameters were taken from the marginal vein of the rabbits’ ears using the free-falling drops method. In order to obtain plasma rich in thrombocytes the blood was centrifuged for 10 minutes at 1000 revolutions per minute, after which the upper layer of plasma was transferred to another test-tube and the remainder was centrifuged again for 20 minutes at 3000 revs/min. to obtain thrombocyte-free plasma.
At one of the stages a study was made of possible changes in the coagulatory system of rabbits in vivo if their ordinary drinking water was replaced by water exposed in the pyramid. The experiments were conducted over 14 days. Blood was taken from the rabbits before they began drinking pyramid water, and also 7 and 14 days into the experiment. The number of thrombocytes was determined by the optical method proposed by B. Walkowiak et al. A sample of rich plasma was dissolved in ten times the amount of a 0.01-molar phosphate buffer, containing 0.14 moles NaCl (pH 7.3) and the density was measured in plastic dishes at A800 against a weak plasma diluted in the same way. The quantity of thrombocytes before the introduction of the pyramid water was taken as 100%. On the 7th day of the experiment the quantity had risen to 129±10% and on the 14th day to 167±18%. A reduction in the prothrombin time and a rise in the number of thrombocytes was reliably established.
Professor V.A. Makarov